The following are a list of protocols used in the Fygenson Lab. Links to those protocols originating from other labs have been provided. Some of the protocols listed have not yet been made available on this website. However, those interested should contact the lab for further information.
Cleaning Glass Slides and Coverslips (PDF)
Common Buffers (courtesy of Salmon Lab, UNC) (URL)
Making Microtubules in Vesicles (PDF)
Negative Stain TEM of Microtubules (courtesy of Mitchison Lab, HMS) (URL)
Plasmid Purification (Qiagen) (PDF)
Preparation of Samples for Light Microscopy (PDF)
Preparation of PVP-coated Glass Slides and Coverslips (PDF) based on "Cross-Linked Polymer Coatings for Capillary Electrophoresis and Application to Analysis of Basic Proteins, Acidic Proteins, and Inorganic Ions" by Kannan Srinivasan,*Chris Pohl, and, and Nebojsa Avdalovic, Analytical Chemistry 1997 69 (14), 2798-2805 link at pubs.acs.org/doi/pdf/10.1021/ac970041m
QuikChange Site-Directed Mutagenesis Kit (Stratagene) (PDF)
Tubulin Basics (courtesy of Mitchison Lab, HMS) (URL)
Whole Cell Yeast PCR Kit (BIO 101) (PDF)
Yeast Tubulin-6xHis Prep (PDF)
Bacteriophage Culture and Storage
Basic Protein Concentration Measurement
E. Coli Culture and Storage
Preparation of Samples for AFM*
Stationary Laser Tweezer Setup
Yeast Culture and Storage